Apoptosis induced by the SARS-associated coronavirus in Vero cells is replication-dependent and involves caspase.
Identifieur interne : 004924 ( Main/Exploration ); précédent : 004923; suivant : 004925Apoptosis induced by the SARS-associated coronavirus in Vero cells is replication-dependent and involves caspase.
Auteurs : Lili Ren ; Renquan Yang ; Li Guo ; Jianguo Qu ; Jianwei Wang ; Tao HungSource :
- DNA and cell biology [ 1044-5498 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Protéines proto-oncogènes c-bcl-2.
- pathogénicité : Virus du SRAS.
- physiologie : Virus du SRAS.
- Animaux, Apoptose, Benzimidazoles, Caspase-3, Cellules Vero, Colorants fluorescents, Fragmentation de l'ADN, Humains, Inhibiteurs des caspases, Protéine Bax, Réplication virale.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Proto-Oncogene Proteins c-bcl-2.
- chemical : Benzimidazoles, Caspase 3, Caspase Inhibitors, Fluorescent Dyes, bcl-2-Associated X Protein.
- pathogenicity : SARS Virus.
- physiology : SARS Virus.
- Animals, Apoptosis, Chlorocebus aethiops, DNA Fragmentation, Humans, Vero Cells, Virus Replication.
Abstract
The pathogenesis of the severe acute respiratory syndrome (SARS), a newly emerging life-threatening disease in humans, remains unknown. It is believed that the modulation of apoptosis is relevant to diseases that are caused by various viruses. To examine potential apoptotic mechanisms related to SARS, we investigated features of apoptosis induced by the SARS-associated coronavirus (SARS-CoV) in host cells. The results indicated that the SARS-CoV-induced apoptosis in Vero cells in a virus replication-dependent manner. Additionally, the downregulation of Bcl-2, the activation of casapse 3, as well as the upregulation of Bax were detected, suggesting the involvement of the caspase family and the activation of the mitochondrial signaling pathway. Although there is a positive correlation between apoptosis and virus replication, the latter is not significantly blocked by treatment with the caspase inhibitor z-DEVD-FMK. These preliminary data provide important information on both the pathogenesis and potential antiviral targets of SARS-CoV.
DOI: 10.1089/dna.2005.24.496
PubMed: 16101347
Affiliations:
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Jianwei" uniqKey="Wang J" first="Jianwei" last="Wang">Jianwei Wang</name></author><author><name sortKey="Hung, Tao" sort="Hung, Tao" uniqKey="Hung T" first="Tao" last="Hung">Tao Hung</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16101347</idno><idno type="pmid">16101347</idno><idno type="doi">10.1089/dna.2005.24.496</idno><idno type="wicri:Area/PubMed/Corpus">002594</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002594</idno><idno type="wicri:Area/PubMed/Curation">002594</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002594</idno><idno type="wicri:Area/PubMed/Checkpoint">002821</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002821</idno><idno type="wicri:Area/Ncbi/Merge">001106</idno><idno type="wicri:Area/Ncbi/Curation">001106</idno><idno 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Yang</name></author><author><name sortKey="Guo, Li" sort="Guo, Li" uniqKey="Guo L" first="Li" last="Guo">Li Guo</name></author><author><name sortKey="Qu, Jianguo" sort="Qu, Jianguo" uniqKey="Qu J" first="Jianguo" last="Qu">Jianguo Qu</name></author><author><name sortKey="Wang, Jianwei" sort="Wang, Jianwei" uniqKey="Wang J" first="Jianwei" last="Wang">Jianwei Wang</name></author><author><name sortKey="Hung, Tao" sort="Hung, Tao" uniqKey="Hung T" first="Tao" last="Hung">Tao Hung</name></author></analytic><series><title level="j">DNA and cell biology</title><idno type="ISSN">1044-5498</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Benzimidazoles</term><term>Caspase 3</term><term>Caspase Inhibitors</term><term>Chlorocebus aethiops</term><term>DNA Fragmentation</term><term>Fluorescent Dyes</term><term>Humans</term><term>Proto-Oncogene Proteins c-bcl-2 (metabolism)</term><term>SARS Virus (pathogenicity)</term><term>SARS Virus (physiology)</term><term>Vero Cells</term><term>Virus Replication</term><term>bcl-2-Associated X Protein</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Benzimidazoles</term><term>Caspase-3</term><term>Cellules Vero</term><term>Colorants fluorescents</term><term>Fragmentation de l'ADN</term><term>Humains</term><term>Inhibiteurs des caspases</term><term>Protéine Bax</term><term>Protéines proto-oncogènes c-bcl-2 (métabolisme)</term><term>Réplication virale</term><term>Virus du SRAS (pathogénicité)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Proto-Oncogene Proteins c-bcl-2</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Benzimidazoles</term><term>Caspase 3</term><term>Caspase Inhibitors</term><term>Fluorescent Dyes</term><term>bcl-2-Associated X Protein</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines proto-oncogènes c-bcl-2</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Chlorocebus aethiops</term><term>DNA Fragmentation</term><term>Humans</term><term>Vero Cells</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Benzimidazoles</term><term>Caspase-3</term><term>Cellules Vero</term><term>Colorants fluorescents</term><term>Fragmentation de l'ADN</term><term>Humains</term><term>Inhibiteurs des caspases</term><term>Protéine Bax</term><term>Réplication virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The pathogenesis of the severe acute respiratory syndrome (SARS), a newly emerging life-threatening disease in humans, remains unknown. It is believed that the modulation of apoptosis is relevant to diseases that are caused by various viruses. To examine potential apoptotic mechanisms related to SARS, we investigated features of apoptosis induced by the SARS-associated coronavirus (SARS-CoV) in host cells. The results indicated that the SARS-CoV-induced apoptosis in Vero cells in a virus replication-dependent manner. Additionally, the downregulation of Bcl-2, the activation of casapse 3, as well as the upregulation of Bax were detected, suggesting the involvement of the caspase family and the activation of the mitochondrial signaling pathway. Although there is a positive correlation between apoptosis and virus replication, the latter is not significantly blocked by treatment with the caspase inhibitor z-DEVD-FMK. These preliminary data provide important information on both the pathogenesis and potential antiviral targets of SARS-CoV.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Guo, Li" sort="Guo, Li" uniqKey="Guo L" first="Li" last="Guo">Li Guo</name><name sortKey="Hung, Tao" sort="Hung, Tao" uniqKey="Hung T" first="Tao" last="Hung">Tao Hung</name><name sortKey="Qu, Jianguo" sort="Qu, Jianguo" uniqKey="Qu J" first="Jianguo" last="Qu">Jianguo Qu</name><name sortKey="Ren, Lili" sort="Ren, Lili" uniqKey="Ren L" first="Lili" last="Ren">Lili Ren</name><name sortKey="Wang, Jianwei" sort="Wang, Jianwei" uniqKey="Wang J" first="Jianwei" last="Wang">Jianwei Wang</name><name sortKey="Yang, Renquan" sort="Yang, Renquan" uniqKey="Yang R" first="Renquan" last="Yang">Renquan Yang</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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